Bioinformatics evaluation and identification of genes and molecular pathways in steroid-induced osteonecrosis of the femoral head
Background: Steroid-induced osteonecrosis of the femoral head (ONFH) is a typical hip joint illness and is tough to be recognized early. At current, the pathogenesis of steroid-induced ONFH stays unclear, and acknowledged and efficient diagnostic biomarkers are poor. The current research aimed to establish probably necessary genes and signaling pathways concerned in steroid-induced ONFH and examine their molecular mechanisms.
Strategies: Microarray knowledge units GSE123568 (peripheral blood) and GSE74089 (cartilage) have been obtained from the Gene Expression Omnibus database, together with 34 ONFH samples and 14 management samples. Morpheus software program and Venn diagram have been used to establish DEGs and co-expressed DEGs, respectively.
Apart from, we carried out Kyoto Encyclopedia of Genome (KEGG) and gene ontology (GO) pathway enrichment evaluation. We assemble a protein-protein interplay (PPI) community by means of GEO2R and used cytoHubba to divide the PPI community into a number of sub-networks. Moreover, quantitative real-time polymerase chain response (qRT-PCR) was carried out to confirm the bioinformatics evaluation outcomes.
Outcomes: A complete of 118 intersecting DEGs have been obtained between the peripheral blood and cartilage samples, together with 40 upregulated genes and 78 downregulated genes. Then, GO and KEGG pathway enrichment evaluation revealed that upregulated DEGs centered on the signaling pathways associated to staphylococcus aureus an infection, leishmaniasis, antigen processing, and presentation, in addition to bronchial asthma and graft-versus-host illness.
Downregulated genes have been concentrated within the FoxO signaling pathway, AMPK signaling pathway, signaling pathway regulating stem cell pluripotency, and mTOR signaling pathway. Some hub genes with excessive interactions comparable to CXCR1, FPR1, MAPK1, FOXO3, FPR2, CXCR2, and TYROBP have been recognized within the PPI community.
The outcomes of qRT-PCR demonstrated that CXCR1, FPR1, and TYROBP have been upregulated whereas MAPK1 was downregulated in peripheral blood of steroid-induced ONFH sufferers. This was in line with the bioinformatics evaluation.
SF1 (Ab-82) Antibody |
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| CSB-PA963117-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against SF1 (Ab-82). Recognizes SF1 (Ab-82) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
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CCT7 Antibody |
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| CSB-PA963179- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against CCT7. Recognizes CCT7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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CCT7 Antibody |
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| CSB-PA963179-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against CCT7. Recognizes CCT7 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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Phospho-MAPT (Ser404) Antibody |
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| CSB-PA963344- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-MAPT (Ser404). Recognizes Phospho-MAPT (Ser404) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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Phospho-MAPT (Ser404) Antibody |
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| CSB-PA963344-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-MAPT (Ser404). Recognizes Phospho-MAPT (Ser404) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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CST1 Antibody |
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| CSB-PA963535- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against CST1. Recognizes CST1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
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CST1 Antibody |
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| CSB-PA963535-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against CST1. Recognizes CST1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IF;IHC:1:50-1:100, IF:1:100-1:500 |
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Phospho-CCND1 (Ser90) Antibody |
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| CSB-PA963558- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-CCND1 (Ser90). Recognizes Phospho-CCND1 (Ser90) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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Phospho-CCND1 (Ser90) Antibody |
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| CSB-PA963558-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-CCND1 (Ser90). Recognizes Phospho-CCND1 (Ser90) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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Histone H3K27me3 Antibody |
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| CSB-PA963638- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Histone H3K27me3. Recognizes Histone H3K27me3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP, ChIP;WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
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Histone H3K27me3 Antibody |
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| CSB-PA963638-100ul | Cusabio | 100ul | EUR 380 |
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Description: A polyclonal antibody against Histone H3K27me3. Recognizes Histone H3K27me3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF, IP, ChIP;WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, IP:1:50-1:200, ChIP:1:50-1:200 |
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MYOM1 Antibody |
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| CSB-PA964006- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against MYOM1. Recognizes MYOM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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MYOM1 Antibody |
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| CSB-PA964006-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against MYOM1. Recognizes MYOM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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ADD3 Antibody |
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| CSB-PA964364- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ADD3. Recognizes ADD3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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ADD3 Antibody |
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| CSB-PA964364-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ADD3. Recognizes ADD3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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TP53 (Ab-315) Antibody |
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| CSB-PA964395- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against TP53 (Ab-315). Recognizes TP53 (Ab-315) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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TP53 (Ab-315) Antibody |
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| CSB-PA964395-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against TP53 (Ab-315). Recognizes TP53 (Ab-315) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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PRLR Antibody |
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| CSB-PA964526- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PRLR. Recognizes PRLR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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PRLR Antibody |
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| CSB-PA964526-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PRLR. Recognizes PRLR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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PPP2R3C Antibody |
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| CSB-PA964594- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PPP2R3C. Recognizes PPP2R3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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PPP2R3C Antibody |
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| CSB-PA964594-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PPP2R3C. Recognizes PPP2R3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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BAGE2 Antibody |
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| CSB-PA964722- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against BAGE2. Recognizes BAGE2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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BAGE2 Antibody |
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| CSB-PA964722-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against BAGE2. Recognizes BAGE2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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PIK3R1/PIK3R3 (Ab-467/199) Antibody |
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| CSB-PA965833- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PIK3R1/PIK3R3 (Ab-467/199). Recognizes PIK3R1/PIK3R3 (Ab-467/199) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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PIK3R1/PIK3R3 (Ab-467/199) Antibody |
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| CSB-PA965833-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PIK3R1/PIK3R3 (Ab-467/199). Recognizes PIK3R1/PIK3R3 (Ab-467/199) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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MAP2K1 (Ab-291) Antibody |
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| CSB-PA965933- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against MAP2K1 (Ab-291). Recognizes MAP2K1 (Ab-291) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
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MAP2K1 (Ab-291) Antibody |
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| CSB-PA965933-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against MAP2K1 (Ab-291). Recognizes MAP2K1 (Ab-291) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:1000, IHC:1:50-1:200 |
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Phospho-HSF1 (Ser307) Antibody |
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| CSB-PA966483- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-HSF1 (Ser307). Recognizes Phospho-HSF1 (Ser307) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
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Phospho-HSF1 (Ser307) Antibody |
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| CSB-PA966483-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-HSF1 (Ser307). Recognizes Phospho-HSF1 (Ser307) from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
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ASCL2 Antibody |
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| CSB-PA966616- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ASCL2. Recognizes ASCL2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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ASCL2 Antibody |
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| CSB-PA966616-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ASCL2. Recognizes ASCL2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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HNRNPC Antibody |
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| CSB-PA966696- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against HNRNPC. Recognizes HNRNPC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
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HNRNPC Antibody |
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| CSB-PA966696-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against HNRNPC. Recognizes HNRNPC from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
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GZMH Antibody |
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| CSB-PA966723- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against GZMH. Recognizes GZMH from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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GZMH Antibody |
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| CSB-PA966723-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against GZMH. Recognizes GZMH from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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Phospho-SYT1 (Ser309) Antibody |
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| CSB-PA967008- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-SYT1 (Ser309). Recognizes Phospho-SYT1 (Ser309) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200 |
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Phospho-SYT1 (Ser309) Antibody |
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| CSB-PA967008-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-SYT1 (Ser309). Recognizes Phospho-SYT1 (Ser309) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:200 |
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NRSN1 Antibody |
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| CSB-PA967502- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against NRSN1. Recognizes NRSN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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NRSN1 Antibody |
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| CSB-PA967502-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against NRSN1. Recognizes NRSN1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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RABL2A Antibody |
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| CSB-PA967554- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against RABL2A. Recognizes RABL2A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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RABL2A Antibody |
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| CSB-PA967554-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against RABL2A. Recognizes RABL2A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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HDAC3 Antibody |
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| CSB-PA967736- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against HDAC3. Recognizes HDAC3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
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HDAC3 Antibody |
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| CSB-PA967736-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against HDAC3. Recognizes HDAC3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 |
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AKR1A1 Antibody |
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| CSB-PA967994- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against AKR1A1. Recognizes AKR1A1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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AKR1A1 Antibody |
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| CSB-PA967994-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against AKR1A1. Recognizes AKR1A1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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CLCN4 Antibody |
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| CSB-PA968321- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against CLCN4. Recognizes CLCN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200 |
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CLCN4 Antibody |
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| CSB-PA968321-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against CLCN4. Recognizes CLCN4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200 |
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ATF1 Antibody |
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| CSB-PA968509- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ATF1. Recognizes ATF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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ATF1 Antibody |
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| CSB-PA968509-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ATF1. Recognizes ATF1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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Phospho-MEF2A (Ser408) Antibody |
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| CSB-PA968646- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-MEF2A (Ser408). Recognizes Phospho-MEF2A (Ser408) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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Phospho-MEF2A (Ser408) Antibody |
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| CSB-PA968646-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-MEF2A (Ser408). Recognizes Phospho-MEF2A (Ser408) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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WISP2 Antibody |
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| CSB-PA968927- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against WISP2. Recognizes WISP2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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WISP2 Antibody |
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| CSB-PA968927-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against WISP2. Recognizes WISP2 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000 |
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Phospho-GRIN2B (Tyr1474) Antibody |
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| CSB-PA969425- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-GRIN2B (Tyr1474). Recognizes Phospho-GRIN2B (Tyr1474) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
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Phospho-GRIN2B (Tyr1474) Antibody |
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| CSB-PA969425-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-GRIN2B (Tyr1474). Recognizes Phospho-GRIN2B (Tyr1474) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200 |
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PMAIP1 Antibody |
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| CSB-PA969433- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PMAIP1. Recognizes PMAIP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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PMAIP1 Antibody |
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| CSB-PA969433-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PMAIP1. Recognizes PMAIP1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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Phospho-ZAP70 (Tyr319) Antibody |
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| CSB-PA969675- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-ZAP70 (Tyr319). Recognizes Phospho-ZAP70 (Tyr319) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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Phospho-ZAP70 (Tyr319) Antibody |
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| CSB-PA969675-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-ZAP70 (Tyr319). Recognizes Phospho-ZAP70 (Tyr319) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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RPS4X Antibody |
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| CSB-PA969688- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against RPS4X. Recognizes RPS4X from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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RPS4X Antibody |
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| CSB-PA969688-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against RPS4X. Recognizes RPS4X from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:3000, IF:1:100-1:500 |
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TUBB3 Antibody |
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| CSB-PA969690- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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TUBB3 Antibody |
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| CSB-PA969690-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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NT5C1B Antibody |
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| CSB-PA969848- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against NT5C1B. Recognizes NT5C1B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
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NT5C1B Antibody |
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| CSB-PA969848-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against NT5C1B. Recognizes NT5C1B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
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AKT1 (Ab-450) Antibody |
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| CSB-PA969941- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
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AKT1 (Ab-450) Antibody |
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| CSB-PA969941-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 |
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Phospho-MAP2K4 (Ser80) Antibody |
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| CSB-PA970074- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against Phospho-MAP2K4 (Ser80). Recognizes Phospho-MAP2K4 (Ser80) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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Phospho-MAP2K4 (Ser80) Antibody |
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| CSB-PA970074-100ul | Cusabio | 100ul | EUR 362 |
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Description: A polyclonal antibody against Phospho-MAP2K4 (Ser80). Recognizes Phospho-MAP2K4 (Ser80) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:100, IF:1:100-1:200 |
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RAB29 Antibody |
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| CSB-PA970360- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against RAB29. Recognizes RAB29 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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RAB29 Antibody |
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| CSB-PA970360-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against RAB29. Recognizes RAB29 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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KIR2DL5B Antibody |
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| CSB-PA970470- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against KIR2DL5B. Recognizes KIR2DL5B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
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KIR2DL5B Antibody |
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| CSB-PA970470-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against KIR2DL5B. Recognizes KIR2DL5B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF;IF:1:100-1:500 |
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IKZF3 Antibody |
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| CSB-PA970658- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against IKZF3. Recognizes IKZF3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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IKZF3 Antibody |
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| CSB-PA970658-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against IKZF3. Recognizes IKZF3 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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USP13 Antibody |
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| CSB-PA970742- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against USP13. Recognizes USP13 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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USP13 Antibody |
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| CSB-PA970742-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against USP13. Recognizes USP13 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;WB:1:500-1:3000, IHC:1:50-1:100 |
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ABCD4 Antibody |
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| CSB-PA970746- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against ABCD4. Recognizes ABCD4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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ABCD4 Antibody |
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| CSB-PA970746-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against ABCD4. Recognizes ABCD4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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AATF Antibody |
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| CSB-PA970851- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against AATF. Recognizes AATF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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AATF Antibody |
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| CSB-PA970851-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against AATF. Recognizes AATF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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MOB1B Antibody |
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| CSB-PA970943- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against MOB1B. Recognizes MOB1B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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MOB1B Antibody |
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| CSB-PA970943-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against MOB1B. Recognizes MOB1B from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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PLG Antibody |
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| CSB-PA970985- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against PLG. Recognizes PLG from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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PLG Antibody |
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| CSB-PA970985-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against PLG. Recognizes PLG from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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OXR1 Antibody |
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| CSB-PA971107- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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OXR1 Antibody |
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| CSB-PA971107-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against OXR1. Recognizes OXR1 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;IHC:1:50-1:100 |
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NBPF5P Antibody |
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| CSB-PA971232- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against NBPF5P. Recognizes NBPF5P from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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NBPF5P Antibody |
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| CSB-PA971232-100ul | Cusabio | 100ul | EUR 316 |
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Description: A polyclonal antibody against NBPF5P. Recognizes NBPF5P from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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KCNJ4 Antibody |
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| CSB-PA971432- | Cusabio | EUR 335 | |
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Description: A polyclonal antibody against KCNJ4. Recognizes KCNJ4 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000 |
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Affiliation analysis of the surfactant protein-C gene to childhood bronchial bronchial asthma
Targets: This analysis targets to clarify the molecular variability throughout the SFTPC gene in a childhood persistent respiratory sickness, bronchial bronchial asthma, throughout the Tunisian inhabitants and to determine the implications based on a case-control analysis of p.Thr138Asn (T138N) and p.Ser186Asn (S186N) variants.
Methods: We used direct sequencing for the genotyping of the SFTPC gene inside 101 asthmatic children. The analysis of T138N and S186N variants in 110 controls is carried out by the PCR-RFLP methodology. Outcomes: The molecular analysis revealed 26 variants along with 24 intronic variations and a pair of exonic variations (T138N and S186N) with respective frequencies of 16.8% and 18.3%. We carried out a case-control analysis of the two acknowledged exonic
variations. A particular genotypic and allelic distribution between the two groups was well-known. Solely the T138N polymorphism confirmed a serious affiliation with bronchial bronchial asthma sickness (p < 10-3).
Statistical analysis elaborated Four haplotypes with the following frequencies in victims vs controls: 138Thr-186Ser (79.5% vs 57.6%), 138Thr-186Asn (3.7% vs 7.8%), 138Asn-186Thr (2.2% vs 20.2%) and 138Asn-186Asn (14.6% vs 14.4%). A serious distinction (p < 10-3) was highlighted in haplotype distribution.
The 138Asn-186Ser (OR [95%CI] = 0.14[0.04-0.54], p = 0.004, R2=0.93) and 138Thr-186Asn (OR [95%CI] = 0.35[0.12-0.54], p = 0.047, R2=0.88) haplotypes confirmed a dangerous affiliation with bronchial bronchial asthma which might signify a defending challenge in opposition to the sickness.
Conclusion: In Tunisia, this work constitutes the first report throughout the SFTPC gene and highlights the genetic variability of the SFTPC gene in bronchial bronchial asthma. On account of this truth, the case-controls analysis may be useful throughout the analysis of surfactant proteins dysfunction in persistent respiratory sickness at an early age.
The genetic foundation of pure antibody titers of younger wholesome pigs and relationships with illness resilience
Background: Sickness resilience is the pliability to maintain up effectivity beneath pathogen publicity nonetheless is troublesome to choose for on account of breeding populations are raised beneath extreme properly being. Selection for resilience requires a trait that is heritable, simple to measure on healthful animals, and genetically correlated with resilience.
Pure antibodies (NAb) are important parts of the innate immune system and are found to be heritable and associated to sickness susceptibility in dairy cattle and poultry. Our purpose was to investigate NAb and complete IgG in blood of healthful, youthful pigs as potential indicator traits for sickness resilience.
Outcomes: Info have been from Yorkshire x Landrace pigs, with IgG and IgM NAb (Four antigens) and complete IgG measured by ELISA in blood plasma collected ~ 1 week after weaning, earlier to their publicity to a pure polymicrobial downside.
Heritability estimates have been lower for IgG NAb (0.12 to 0.24, + 0.05) and for complete IgG (0.19 + 0.05) than for IgM NAb (0.33 to 0.53, + 0.07) nonetheless maternal outcomes have been larger for IgG NAb (0.41 to 0.52, + 0.03) and for complete IgG (0.19 + 0.05) than for IgM NAb (0.00 to 0.10, + 0.04).
Phenotypically, IgM NAb titers have been moderately correlated with each other (widespread 0.60), as have been IgG NAb titers (widespread 0.42), nonetheless correlations between IgM and IgG NAb titers have been weak (widespread 0.09). Phenotypic correlations of complete IgG have been cheap with NAb IgG (widespread 0.46) nonetheless weak with NAb IgM (widespread 0.01).
Estimates of genetic correlations amongst NAb confirmed associated patterns nonetheless with small SE, with estimates averaging 0.76 amongst IgG NAb, 0.63 amongst IgM NAb, 0.17 between IgG and IgM NAb, 0.64 between complete IgG and IgG NAb, and 0.13 between complete IgG and IgM NAb. Phenotypically, pigs that survived had barely elevated ranges of NAb and complete IgG than pigs that died.
Genetically, elevated ranges of NAb tended to be associated to raised sickness resilience based on lower mortality and fewer parenteral antibiotic therapies. Genome-wide affiliation analyses for NAb titers acknowledged a lot of genomic areas, with a lot of candidate genes for immune response.
Conclusions: Ranges of NAb in blood of healthful youthful piglets are heritable and potential genetic indicators of resilience to polymicrobial sickness.